MOLECULAR-GENETIC MECHANISMS OF RESISTANCE OF NOSOCOMIAL KLEBSIELLA PNEUMONIAE STRAINS TO POLYMYXINS AND ANTIBIOTICS OF OTHER GROUPS ACCORDING TO WHOLE GENOME SEQUENCING DATA

Identification of numerous mechanisms resistance to colistin and other antibiotics is possible using whole genome sequencing. Objectives. To assess the molecular-genetic polymyxins groups in nosocomial Klebsiella pneumoniae strains. Material methods. For 13 multidrug- extensively drug-resistant K.pneumoniae strains semiconductor sequencing was performed Ion PGM System genomic sequencer (Thermo Fisher Scientific, USA). The assembly sequences their annotation were carried out. PROVEAN software tool used predict influence nucleotide replacements on structure amino acid functional activity proteins. identification antibiotic genes search for efflux by ResFinder v.4.1 CARD web resources. Results. Several types β-lactamase detected simultaneously all strains, as well fosfomycin. Genes aminoglycosides identified 11 chloramphenicol - 10, rifampicin 5, macrolides 4. mcr phosphoethanolamine transferase absent Functionally significant substitutions revealed pmrB gene (D150Y, T157P, G207S) comparing studied samples with reference K. strain ATCC 700603. Changes mgrB also found colistin-resistant (W20R replacement, insertional inactivation transposons IS1, IS4 IS5 families). Conclusions. results represent majority including β-lactams, aminoglycosides, fluoroquinolones, fosfomycin, chloramphenicol, polymyxins. Genetic determinants (insertional deletion gene; D150Y, T157P G207S gene) MIC 64-128 mg/l absence colistin-susceptible